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Related Experiment Videos

Apical Na+ conductance in maturing rabbit principal cell

L M Satlin1, L G Palmer

  • 1Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

The American Journal of Physiology
|March 1, 1996
PubMed
Summary

Neonatal rabbits show low sodium absorption due to fewer and less active apical sodium channels in principal cells. Channel number and open probability increase significantly after birth, enhancing sodium transport.

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Area of Science:

  • Nephrology
  • Renal Physiology
  • Cellular Biology

Background:

  • Net sodium (Na+) absorption in rabbit cortical collecting ducts (CCDs) is low in the first week of postnatal life.
  • This low absorption rate may be linked to reduced apical Na+ permeability in neonatal principal cells.

Purpose of the Study:

  • To investigate whether low Na+ absorption post-birth is due to low apical Na+ channel activity in neonatal principal cells.
  • To estimate the conductance, number (N), and open probability (Po) of these channels in maturing rabbits.

Main Methods:

  • Utilized the patch-clamp technique on split-open CCDs from rabbits of different postnatal ages (1, 2, and 5 weeks).
  • Measured inward currents, channel conductance, N, and Po under specific ionic and temperature conditions.

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  • Confirmed the identity of the channel as amiloride-sensitive by observing amiloride's effect on channel activity.

    • Main Results:

    • An 11-pS conductance channel was observed, with its detection rate increasing from 17% at 1 week to 41% at 2 weeks and 43% at 5 weeks.
    • The mean number of channels per patch (N) was significantly lower in the 1st week (0.22) compared to the 2nd (1.38) and 5th (1.24) weeks.
    • The open probability (Po) was significantly lower in the 1st week (0.085) than in the 2nd (0.345) and 5th weeks (0.291).
    • Amiloride (0.5 microM) caused >90% reduction in channel activity, confirming it as an amiloride-sensitive Na+ channel.

    Conclusions:

    • The number and open probability of apical amiloride-sensitive Na+ channels in principal cells increase significantly after birth.
    • This developmental increase in channel activity contributes to the enhanced net Na+ absorption observed in older rabbits.