Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease of glomerular disialogangliosides in puromycin nephrosis of the rat

H Holthöfer1, J Reivinen, M L Solin

  • 1Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Finland.

The American Journal of Pathology
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Substantial variability in morphological scaling among bumblebee colonies.

Royal Society open science·2022
Same author

Publisher Correction: X-Ray Nanotomography of Individual Pulp Fibre Bonds Reveals the Effect of Wall Thickness on Contact Area.

Scientific reports·2020
Same author

Revealing interfacial disorder at the growth-front of thick many-layer epitaxial graphene on SiC: a complementary neutron and X-ray scattering investigation.

Nanoscale·2019
Same author

X-Ray Nanotomography of Individual Pulp Fibre Bonds Reveals the Effect of Wall Thickness on Contact Area.

Scientific reports·2019
Same author

Attitudes of anonymous and identity-release oocyte donors towards future contact with donor offspring.

Human reproduction (Oxford, England)·2019
Same author

Podocyte apoptosis is prevented by blocking the Toll-like receptor pathway.

Cell death & disease·2015

Puromycin aminonucleoside nephrosis (PAN) in rats shows a significant decrease in glomerular O-acetyl GD3 and GD3 gangliosides. These changes precede proteinuria, suggesting a role in minimal change nephropathy development.

Area of Science:

  • Nephrology
  • Biochemistry
  • Immunohistochemistry

Background:

  • Puromycin aminonucleoside nephrosis (PAN) is a rat model for human minimal change nephropathy.
  • Defective sialylation of podocyte sialoprotein podocalyxin is known in PAN.
  • The role of glomerular glycosphingolipid sialylation in PAN remains unclear.

Purpose of the Study:

  • To investigate changes in glomerular gangliosides, specifically GD3 and O-acetyl GD3, during acute PAN.
  • To compare these changes with another rat model, Heymann nephritis.
  • To determine if altered ganglioside sialylation is linked to proteinuria in PAN.

Main Methods:

  • Sequential immunohistochemical and biochemical analysis of rat glomeruli.
  • Analysis of gangliosides GD3 and O-acetyl GD3 in PAN and Heymann nephritis models.

Related Experiment Videos

  • Dose-dependent and time-course studies following puromycin injection.

    • Main Results:

    • A marked decrease in glomerular O-acetyl GD3 and GD3 was observed in PAN, peaking at 10 days post-puromycin.
    • These ganglioside reductions occurred before the onset of overt proteinuria and were dose-dependent.
    • No similar changes were found in glomeruli of Heymann nephritic rats.

    Conclusions:

    • Puromycin-induced nephrosis in rats involves a significant reduction in glomerular GD3 and O-acetyl GD3.
    • The observed decrease in gangliosides precedes proteinuria, suggesting a potential causal role in PAN.
    • The defect appears specific to puromycin action, not a general consequence of proteinuria.