Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

gp300sca is not a high affinity Notch ligand

E C Lee1, N E Baker

  • 1Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Biochemical and Biophysical Research Communications
|August 23, 1996
PubMed
Summary

The secreted protein scabrous (sca) does not bind to the Notch receptor, suggesting it acts through a different mechanism in cell determination and differentiation. This finding impacts understanding of developmental signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of poor maternal diet during gestation are detected in F2 offspring.

Translational animal science·2024
Same author

A Single Dose of Ibuprofen Impacts IL-10 Response to 164-km Road Cycling in the Heat.

Research quarterly for exercise and sport·2022
Same author

Racial disparities after stoma construction in colorectal surgery.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2019
Same author

Effects of Alzheimer's disease and Lewy body disease on subcortical atrophy.

European journal of neurology·2019
Same author

Living-donor Liver Retransplantation Using the Vessels of the Previous Allograft by Means of Intragraft Dissection.

Transplantation proceedings·2018
Same author

Randomized clinical trial of preoperative skin antisepsis with chlorhexidine gluconate or povidone-iodine.

The British journal of surgery·2016

Area of Science:

  • Developmental biology
  • Cell signaling
  • Neuroscience

Background:

  • The Notch protooncogene is crucial for cell determination and differentiation across species.
  • The secreted protein scabrous (sca) was hypothesized to be a Notch ligand in the peripheral nervous system.

Purpose of the Study:

  • To investigate the potential interaction between scabrous (sca) and the Notch receptor.
  • To clarify the mechanism of action for scabrous in cellular development.

Main Methods:

  • Purification of the scabrous (sca) protein.
  • Utilizing a cell line engineered to express high levels of Notch receptors (18,000 per cell).
  • Employing coimmunoprecipitation, cell adhesion assays, and binding assays with labeled scabrous (sca).

Related Experiment Videos

Main Results:

  • No detectable interaction was observed between purified gp300sca and Notch receptors.
  • No interaction was found between gp300sca and the related Delta protein.
  • These results indicate scabrous does not function as a direct Notch ligand.

Conclusions:

  • Scabrous (sca) likely mediates its effects on cell determination and differentiation through a mechanism independent of direct Notch receptor binding.
  • Further research is needed to elucidate the precise signaling pathway utilized by scabrous.