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Related Experiment Videos

Nitric oxide interactions with cobalamins: biochemical and functional consequences

M Brouwer1, W Chamulitrat, G Ferruzzi

  • 1Duke University Marine Biomedical Center, Beaufort, NC, USA.

Blood
|September 1, 1996
PubMed
Summary

Nitric oxide (NO) interacts with vitamin B12 derivatives, particularly aquocobalamin, forming stable nitrosylcobalamin. This complex can transfer NO and impacts biological functions, affecting enzymes and cell proliferation.

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Area of Science:

  • Biochemistry
  • Chemical Biology

Background:

  • Nitric oxide (NO) is a key signaling molecule involved in numerous biological processes.
  • NO often exerts its functions by forming nitrosyl complexes with heme proteins.
  • The interaction of NO with vitamin B12 derivatives remains incompletely understood.

Purpose of the Study:

  • To investigate the interactions between nitric oxide (NO) and various cobalt-containing vitamin B12 derivatives.
  • To characterize the resulting complexes and their chemical properties.
  • To explore the functional consequences of these interactions in biological systems.

Main Methods:

  • Absorption spectroscopy to study NO binding to cobalamins.
  • Electron paramagnetic resonance (EPR) spectroscopy to identify radical species.

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  • Photolysis experiments to investigate complex stability and NO transfer.
  • Enzyme cofactor activity assays and cell proliferation studies.

    • Main Results:

    • Aquocobalamin (H2O-Cbl) was the only Co(III) derivative that directly combined with NO, forming a stable Co(III)-NO complex.
    • This complex could transfer its NO moiety to hemoglobin and glutathione.
    • Photolysis of other cobalamins (Ado-Cbl, Me-Cbl) in the presence of NO led to NO-Co(II) formation.
    • Nitrosylcobalamin exhibited reduced cofactor activity for methionine synthase and could inhibit NO-mediated effects on cell proliferation.

    Conclusions:

    • Aquocobalamin readily interacts with nitric oxide, forming stable nitrosylcobalamin complexes.
    • These interactions have significant functional implications, including altered enzyme activity and modulation of cellular responses to NO.
    • Cobalamins play a role in modulating NO signaling pathways in vivo.