Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative relation between interstitial adenosine concentration and coronary blood flow

D W Stepp1, R Van Bibber, K Kroll

  • 1Department of Physiology and Biophysics, University of Washington, Seattle 98195-7290, USA.

Circulation Research
|September 1, 1996
PubMed
Summary

Basal interstitial adenosine levels are near the threshold for increasing coronary blood flow. Small changes in adenosine concentration significantly impact flow, highlighting the need for careful interpretation of measurements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endothelial ENaC as a repressor of oxidative stress and a guardian of lung capillary barrier function in bacterial and viral pneumonia.

Frontiers in physiology·2025
Same author

Adult acute myeloid leukemia with trisomy 11 as the sole abnormality is characterized by the presence of five distinct gene mutations: MLL-PTD, DNMT3A, U2AF1, FLT3-ITD and IDH2.

Leukemia·2016
Same author

Impaired NO-dependent dilation of skeletal muscle arterioles in hypertensive diabetic obese Zucker rats.

American journal of physiology. Heart and circulatory physiology·2001
Same author

Nitric oxide limits coronary vasoconstriction by a shear stress-dependent mechanism.

American journal of physiology. Heart and circulatory physiology·2001
Same author

Altered serum sex steroids and vitellogenin induction in walleye (Stizostedion vitreum) collected near a metropolitan sewage treatment plant.

Archives of environmental contamination and toxicology·2001
Same author

Alteration of cardiovascular and neuronal function in M1 knockout mice.

Life sciences·2001

Area of Science:

  • Cardiovascular Physiology
  • Pharmacology

Background:

  • Adenosine plays a crucial role in regulating coronary blood flow.
  • Understanding the precise relationship between adenosine concentration and coronary vasodilation is vital for cardiovascular research.

Purpose of the Study:

  • To investigate the dose-response relationship between interstitial adenosine and coronary blood flow.
  • To determine if adenosine-mediated coronary vasodilation involves an endothelial mechanism in vivo.

Main Methods:

  • Utilized a mathematical model of the myocardium to estimate interstitial adenosine concentrations.
  • Infused exogenous adenosine and manipulated endogenous adenosine levels in anesthetized dogs.
  • Measured coronary flow responses to varying adenosine concentrations.

Related Experiment Videos

Main Results:

  • Basal interstitial adenosine concentration was found to be at the threshold for coronary flow increase.
  • A modest increase in interstitial adenosine (62%) sufficed to elevate coronary flow significantly (5% to 50% of maximal).
  • No significant difference was observed in the ED50 for exogenous versus endogenous adenosine, refuting an additional endothelial dilator effect.

Conclusions:

  • A steep dose-response curve exists for adenosine's effect on coronary blood flow.
  • Adenosine-mediated coronary vasodilation in vivo does not appear to involve a secondary endothelial mechanism.
  • Measurements of adenosine concentration require cautious interpretation due to the steep dose-response relationship.