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Molecular cloning of the human rad gene: gene structure and complete nucleotide sequence

J S Caldwell1, J S Moyers, A Doria

  • 1Research Division, Joslin Diabetes Center, Boston, MA, USA.

Biochimica Et Biophysica Acta
|August 23, 1996
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...