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A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl beta-D-lactoside derivatives
L X Wang1, N V Pavlova, S C Li
1Department of Biology, Johns Hopkins University, Baltimore, MD 21218-2685, USA.
Glycoconjugate Journal
|June 1, 1996
Abstract:
4-Methylumbelliferyl 6'-O-benzyl-beta-D-lactoside (6'Bn-MU-Lac) and some related compounds were synthesized via different selective reactions including phase-transfer glycosylation. Their suitability as substrates for a fluorometric assay of ceramide glycanase (CGase) was evaluated. Among others, the 6'Bn-MU-Lac, which is resistant to exogalactosidase, was found to be a suitable substrate for routine assay of the CGase activity. For American leech CGase, the K(m) value is 0.232 mM at pH 5.