Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electron microscopic studies on Vibrio cholerae O139

S Garg1, A N Ghosh, A K Mukhopadhyay

  • 1National Institute of Cholera & Enteric Diseases, Calcutta.

The Indian Journal of Medical Research
|July 1, 1996
PubMed
Summary

Vibrio cholerae O139 possesses a unique capsule, distinguishing it from V. cholerae O1. This difference in surface architecture, including unique epitopes, was identified using electron microscopy and immunoelectron microscopy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of growth and virulence of Vibrio cholerae by carvacrol, an essential oil component of Origanum spp.

Journal of applied microbiology·2021
Same author

Testing the efficacy and safety of BIO101, for the prevention of respiratory deterioration, in patients with COVID-19 pneumonia (COVA study): a structured summary of a study protocol for a randomised controlled trial.

Trials·2021
Same author

Intestinal microbiota and vaccine efficacy in children from resource poor settings - potential impact for the usefulness of probiotics?

Beneficial microbes·2020
Same author

Alterations in glucose metabolism in Vibrio cholerae serogroup O1 El Tor biotype strains.

Scientific reports·2020
Same author

Genomic and Proteomic Characterizations of <i>Sfin-1</i>, a Novel Lytic Phage Infecting Multidrug-Resistant <i>Shigella</i> spp. and <i>Escherichia coli</i> C.

Frontiers in microbiology·2019
Same author

Identification of common virulence factors present in enterotoxigenic Escherichia coli isolated from diarrhoeal patients in Kolkata, India.

Journal of applied microbiology·2018

Area of Science:

  • Microbiology
  • Bacteriology
  • Surface Science

Background:

  • Vibrio cholerae (V. cholerae) is a Gram-negative bacterium responsible for cholera.
  • Distinguishing between different serogroups of V. cholerae is crucial for epidemiological studies and understanding virulence.
  • The surface architecture of V. cholerae O139 has not been extensively characterized in comparison to other serogroups.

Purpose of the Study:

  • To investigate and characterize the surface architecture of V. cholerae O139.
  • To compare the surface features of V. cholerae O139 with V. cholerae O1 and other serogroups.
  • To identify unique surface markers for the V. cholerae O139 serogroup.

Main Methods:

  • Electron microscopy (EM) was used to examine the general morphology and surface structures like pili.

Related Experiment Videos

  • High-power EM provided detailed visualization of pili dimensions and density.
  • Immunoelectron microscopy (IEM) with a specific anti-O139 monoclonal antibody was employed to detect O139-specific antigens.

    • Main Results:

    • V. cholerae O139 shares general morphological features with V. cholerae O1, including a comma shape and a single polar flagellum.
    • Electron microscopy revealed the presence of pili (5-7 nm diameter) on V. cholerae O139.
    • Crucially, V. cholerae O139 was found to possess a capsule, absent in V. cholerae O1, clearly visualized by EM after polycationic ferritin treatment.
    • IEM confirmed that an anti-O139 monoclonal antibody bound exclusively to O139 strains, indicating unique O139 epitopes.

    Conclusions:

    • V. cholerae O139 is morphologically similar to V. cholerae O1 biotypes but is distinguished by the presence of an outer capsule.
    • Unique epitopes on the surface of V. cholerae O139 were identified, differentiating it from other V. cholerae serogroups.
    • These distinct surface characteristics, particularly the capsule and unique epitopes, can aid in the identification and differentiation of V. cholerae O139.