Volume sensitive 36Cl fluxes in L6 myoblasts

Efflux of 36Cl ions was followed in undifferentiated L6 myoblasts at rest and after exposure to hypotonic saline in order to test participation of the chloride exit in regulatory volume decrease mechanism. The efflux curve can be fitted at least with two time constants. After the hypotonic stress the slow rate constant increases almost threefold, from 0.021 +/- 0.019 min-1 normal saline to 0.060 +/- 0.019 min-1 in hypotonic solution.