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Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance
P A Haynes1, D Sheumack, J Kibby
1School of Chemistry, Macquarie University, North Ryde, Australia.
Journal of Chromatography
|March 1, 1991
Abstract:
A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.