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Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes
J A Pérez-Gonzalez1, L H De Graaff, J Visser
1Departmento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos, Burjassot, Valencia, Spain.
Applied and Environmental Microbiology
|June 1, 1996
Abstract:
Two Aspergillus nidulans genes, xlnA and xlnB, encoding the X22 and X24 xylanases from this fungus, respectively, have been cloned and sequenced. Their cDNAs have been expressed in a laboratory Saccharomyces cerevisiae strain under the control of a constitutive yeast promoter, resulting in the construction of recombinant xylanolytic yeast strains.