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Detection of immobilized amplicons by ELISA-like techniques
A A Oroskar1, S E Rasmussen, H N Rasmussen
1Nalge Nunc International, Naperville, IL 60563-1796, USA.
Clinical Chemistry
|September 1, 1996
Abstract:
The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.