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Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast
N Inagaki1, H Kawasaki, H Nagai
1Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Abstract:
Histamine release from purified rat peritoneal mast cells (PMC) was examined and compared to that from a non-purified preparation (PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with compound 48/80, calcium ionophore A23187 and substance P. In contrast, IgE-dependent histamine release from PMC caused by antigen, anti-IgE and concanavalin A was very low compared to that of PEC. The reduced IgE-dependent histamine release from PMC, however, was recovered when PMC was reconstituted with non-mast cells (NMC) present in the peritoneal cavity. The effect was time-dependent and reached a plateau in 30 min. NMC from both sensitized and non-sensitized rats recovered the reduced histamine release from PMC dose-dependently. The potentiating effect of NMC was observed even in the presence of excess amount of phosphatidylserine. Supernatants of NMC and a mixture of PMC and NMC incubated for 1 hr at 37 degrees C, however, failed to potentiate the histamine release. These results demonstrate that IgE-dependent histamine release from rat peritoneal mast cells is upregulated by other cells present in the peritoneal cavity, and that the mechanism involved is distinct from that of phosphatidylserine.
Insights
Non-mast cells in rat peritoneal fluid enhance IgE-dependent histamine release from mast cells. This interaction is crucial for regulating mast cell responses and involves mechanisms beyond phosphatidylserine.
Area of Science:
- Immunology
- Cell Biology
- Allergy Research
Background:
- Mast cells (MC) are key players in allergic reactions, releasing histamine.
- Purified mast cells (PMC) exhibit altered responses compared to non-purified preparations (PEC).
- Understanding mast cell regulation is vital for allergy and inflammation research.
Purpose of the Study:
- To compare histamine release from purified rat peritoneal mast cells (PMC) versus non-purified peritoneal cell exudates (PEC).
- To investigate the role of non-mast cells (NMC) in modulating IgE-dependent histamine release from PMC.
- To elucidate the mechanism underlying NMC-mediated potentiation of mast cell degranulation.
Main Methods:
- Histamine release assays using compound 48/80, calcium ionophore A23187, substance P, antigen, anti-IgE, and concanavalin A.
- Comparison of histamine release from purified mast cells (PMC) and peritoneal cell exudates (PEC).
- Reconstitution experiments involving PMC and non-mast cells (NMC) from rat peritoneal cavities.
Main Results:
- Both PEC and PMC showed similar histamine release with non-IgE-dependent stimuli.
- IgE-dependent histamine release was significantly lower in PMC compared to PEC.
- Non-mast cells (NMC) dose-dependently restored IgE-dependent histamine release from PMC.
- NMC potentiation occurred rapidly (plateau at 30 min) and was independent of phosphatidylserine or soluble factors.
Conclusions:
- Non-mast cells (NMC) in the peritoneal cavity play a crucial role in upregulating IgE-dependent histamine release from rat peritoneal mast cells (PMC).
- The mechanism of NMC-mediated potentiation is distinct from phosphatidylserine effects.
- These findings highlight intercellular communication in regulating mast cell degranulation and allergic responses.