Functional somatotopic organisation of motoneurons supplying the rabbit masseter muscle

The localisation within the trigeminal motor nucleus of motoneurons supplying different regions of the rabbit masseter muscle was investigated to test the hypothesis that muscle regions with different motor tasks are controlled from different subregions of the motor nucleus. Motoneurons were labeled retrogradely with horseradish peroxidase, applied surgically to small sections of the masseter in 22 animals, and also by applying this tracer to the cut masseteric nerve. After sacrifice, the labeled muscle sections were mapped. The distribution of labeled motoneurons within the nucleus was described and compared for the muscle regions. The motoneurons for the masseter muscle are confined to the dorsal and lateral sections of the motor nucleus, along its full rostrocaudal extent. Within this subnucleus, the motoneurons for the superficial masseter occupy the dorsolateral portion, the motoneurons for the deep masseter the dorsomedial portion. The anatomical and functional subdivision of the deep masseter into an anterior and posterior portion appeared to be matched by a separation of the motoneurons for these portions in the rostrocaudal direction along the nucleus. The separation of the motoneurons for the anterior and posterior deep masseter is not complete; the territories in the motor nucleus overlap each other for about 50%. The well-established differentiation in motor tasks between the masseter portions during feeding is thus clearly reflected in a separation of motoneurons, making possible differentiation of descending or afferent input to the separate regions in the nucleus.