Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests

M F Maiden1, A Tanner, P J Macuch

  • 1Department of Periodontal Microbiology, Forsyth Dental Center, Boston, Massachusetts 02115, USA. mmaiden@forsyth.org

Journal of Clinical Microbiology
|February 1, 1996
PubMed
Summary

This study introduces a rapid fluorogenic substrate test for identifying subgingival bacteria. The method accurately differentiates numerous species, aiding in quick presumptive identification of oral pathogens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The pulmonary rehabilitation regimen: a treatment for frailty and 'inflammaging'?

British journal of hospital medicine (London, England : 2005)·2018
Same author

The casual poor and the city of London Poor Law Union, 1837-1869.

Historical journal (Cambridge, England)·2011
Same author

Are epilepsy classifications based on epileptic syndromes and seizure types outdated?

Epileptic disorders : international epilepsy journal with videotape·2006
Same author

Second case report of a metachronous double primary gastric cancer.

British journal of hospital medicine (London, England : 2005)·2005
Same author

Tracing Sydenham's chorea: historical documents from a British paediatric hospital.

Archives of disease in childhood·2005
Same author

Thomas Orme Dudfield: the model medical officer of health.

Journal of medical biography·2001

Area of Science:

  • Microbiology
  • Oral Health
  • Bacterial Identification

Background:

  • Subgingival bacterial identification is crucial for diagnosing and treating periodontal diseases.
  • Accurate and rapid identification methods are needed for clinical microbiology.

Purpose of the Study:

  • To evaluate a panel of fluorogenic substrate tests for the biochemical characterization and identification of subgingival bacteria.
  • To assess the sensitivity and speed of fluorogenic tests for presumptive species-level identification.

Main Methods:

  • Characterization of 892 patient isolates and 121 reference strains representing diverse subgingival bacterial species.
  • Utilized a panel of fluorogenic 4-methylumbelliferyl-linked substrate tests for biochemical profiling.
  • Confirmed isolate identities using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins.

Related Experiment Videos

Main Results:

  • Characteristic fluorogenic reaction profiles differentiated most tested species, including various Porphyromonas, Prevotella, Capnocytophaga, Actinomyces, Propionibacterium, and Streptococcus species.
  • Two mannoside isomers distinguished Actinomyces israelii and Actinomyces gerencseriae.
  • Specific species like Porphyromonas gingivalis, Bacteroides forsythus, and Capnocytophaga species showed characteristic enzyme activities, while others like Fusobacterium nucleatum did not react.

Conclusions:

  • Fluorogenic substrate tests offer a sensitive, simple, and rapid method for biochemical characterization of subgingival bacteria.
  • This approach allows for presumptive species-level identification of most isolates within 4 hours.
  • The method is ideal for rapid presumptive identification before confirmation by definitive techniques like SDS-PAGE.