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New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid
1Institute of Medical Virology, JWG-University Frankfurt, Frankfurt am Main, Germany.
Journal of Clinical Microbiology
|February 1, 1996
Abstract:
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.