Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Iron from complex salts and its bioavailability to rats

J E Dutra-de-Oliveira1, M L Freitas, J F Ferreira

  • 1Department of Internal Medicine, São Paulo University, SP, Brazil.

International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition
|January 1, 1995
PubMed
Summary

Iron bioavailability from complex iron compounds was tested in rats. Sodium iron EDTA and iron glycine chelate showed high iron bioavailability, similar to ferrous sulfate, while ferric orthophosphate had lower bioavailability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Annual Variation in Stallion Testicular Measurements and Its Association With Hormonal Profiles and Semen Quality.

Reproduction in domestic animals = Zuchthygiene·2026
Same author

Managing urethral obstruction in male cats: a practical guide and literature review.

New Zealand veterinary journal·2025
Same author

Use of thermography in the long-term evaluation of scrotal surface temperature and its impact on seminal quality in stallions.

Reproduction in domestic animals = Zuchthygiene·2024
Same author

Predictors of atypical femoral fractures: experience of a Portuguese Fracture Liaison Service.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2024
Same author

Hydrological contraction patterns and duration of drying period shape microbial-mediated litter decomposition.

The Science of the total environment·2021
Same author

DNA methylation pattern changes following a short-term hypocaloric diet in women with obesity.

European journal of clinical nutrition·2020

Area of Science:

  • Nutritional Science
  • Biochemistry
  • Toxicology

Background:

  • Complex iron compounds are increasingly utilized as iron fortifiers.
  • Assessing the bioavailability of iron from these compounds is crucial for human and animal nutrition.
  • Ferrous sulfate is a common reference standard for iron bioavailability studies.

Purpose of the Study:

  • To compare the iron bioavailability of three complex iron compounds (ferric orthophosphate, sodium iron EDTA, iron glycine chelate) against ferrous sulfate.
  • To evaluate the efficacy of these iron complexes as prophylactic iron suppliers in a rat model.
  • To establish a practical and feasible method for testing iron salt bioavailability.

Main Methods:

  • A prophylactic iron study was conducted using weanling rats fed low-iron diets supplemented with different iron compounds.

Related Experiment Videos

  • Diets were fortified to 20 mg Fe/kg with ferrous sulfate, ferric orthophosphate, sodium iron EDTA, or iron glycine chelate.
  • Rats were monitored for 5 weeks, with measurements of weight gain, food intake, and analysis of blood and liver iron content.

    • Main Results:

    • Sodium iron EDTA and iron glycine chelate demonstrated iron bioavailability and biological value comparable to ferrous sulfate.
    • Ferric orthophosphate exhibited a significantly lower biological value for iron compared to ferrous sulfate and the other tested iron complexes.
    • The iron rat assay proved effective for comparing the bioavailability of different iron salts.

    Conclusions:

    • Sodium iron EDTA and iron glycine chelate are effective iron suppliers with high bioavailability in rats.
    • Ferric orthophosphate has a lower iron bioavailability compared to ferrous sulfate and other tested iron complexes.
    • The rat model is a suitable method for evaluating iron bioavailability from various iron compounds.