Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A candidate live inactivatable attenuated vaccine for AIDS

B K Chakrabarti1, R K Maitra, X Z Ma

  • 1Department of Molecular Biology, Cleveland Clinic Foundation, OH 44195, USA.

Proceedings of the National Academy of Sciences of the United States of America
|September 3, 1996
PubMed
Summary

A novel gene-therapy approach for AIDS involves engineering HIV with a suicide gene, making it sensitive to ganciclovir. This strategy aims to create a safer, conditionally live AIDS vaccine by eliminating infected cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research progress in the treatment of maxillary transverse deficiency with combined orthodontic-orthognathic approach].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2024
Same author

[Implantation of a monofocal intraocular lens after femtosecond laser intrastromal keratotomy and contralateral implantation of a trifocal IOL: a case report].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2022
Same author

[Investigation and analysis of perioperative antimicrobials prophylaxis for type Ⅰ incision surgery in a tertiary hospital from 2013 to 2016].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2018
Same author

[Comparisons of clinical effects among three kinds of treatments of 1/3 displaced midshaft clavicular fractures in adults].

Zhonghua yi xue za zhi·2016
Same author

Dominant factor determining the conduction-type of nitrogen-doped ZnO film.

Journal of nanoscience and nanotechnology·2014
Same author

Underlying heart disease and microbiological spectrum of adult infective endocarditis in one Chinese university hospital: a 10-year retrospective study.

Internal medicine journal·2013

Area of Science:

  • Virology
  • Immunology
  • Gene Therapy

Background:

  • Naturally occurring nef-attenuated HIV in nonprogressors suggests potential for live AIDS vaccines.
  • Live attenuated vaccines, particularly those with nef-attenuation, are considered promising candidates for AIDS vaccines.
  • Significant risks are associated with live HIV vaccines, necessitating enhanced safety measures.

Purpose of the Study:

  • To develop a safer, conditionally live vaccine for AIDS by incorporating a conditional lethal genetic element into HIV.
  • To investigate the efficacy of a suicide gene strategy targeting both virus replication and infected cell survival.
  • To assess the stability and sensitivity of engineered viruses to antiviral drugs.

Main Methods:

  • Introduction of the herpes simplex virus thymidine kinase (tk) suicide gene into nef-deleted HIV-1 and SIV molecular clones.

Related Experiment Videos

  • Culturing engineered HIV-tk and SIV-tk in cell lines and primary cultures.
  • Testing the sensitivity of engineered viruses and infected cells to ganciclovir (GCV).
  • Evaluating the stability of the engineered virus against GCV resistance.

    • Main Results:

    • HIV-tk and SIV-tk demonstrated sensitivity to GCV at subtoxic concentrations.
    • GCV effectively eliminated virus-infected cells in both tumor cell lines and primary cultures.
    • The engineered HIV-tk virus exhibited remarkable stability, even after prolonged culture and challenge.
    • While GCV-resistant mutants emerged, a substantial portion of the virus remained susceptible to GCV.

    Conclusions:

    • The engineered HIV-tk virus represents a potentially safer, conditionally live vaccine candidate for AIDS.
    • The suicide gene strategy effectively targets virus replication and infected cell survival, offering enhanced safety.
    • Further research into managing GCV resistance is warranted for clinical application.