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Changes in perimicrovascular protein spatial distribution due to superfusate
B J Barber1, S Dutta, S Parameswaran
1Center for Biomedical Engineering, University of Kentucky, Lexington 40506-0070, USA.
Microcirculation (New York, N.Y. : 1994)
|July 1, 1994
Summary
Superfusion significantly altered plasma protein distribution in the interstitial matrix. Protein concentrations near microvessels decreased, suggesting changes in interstitial gel properties affect protein mobility.
Area of Science:
- Physiology
- Biophysics
- Microcirculation Research
Background:
- The interstitial matrix surrounding microvessels plays a crucial role in regulating fluid and solute exchange.
- Understanding protein distribution within this matrix is essential for comprehending microvascular function and disease pathogenesis.
Purpose of the Study:
- To investigate the impact of superfusion on plasma protein distribution within the perimicrovascular interstitial matrix.
- To quantify changes in protein concentration gradients in response to superfusion.
Main Methods:
- Intravital video microspectrophotometry was employed in anesthetized rats with exteriorized mesenteric loops.
- Analysis focused on spatial distribution of proteins and collagen using specific wavelengths.
- Perimicrovascular protein concentrations were modeled using an exponential decay function.
Main Results:
- Superfusion with normal saline significantly decreased distal protein concentration (ci) and proximal protein concentration (ci + cr).
- Repeated superfusions led to progressive reductions in both ci and ci + cr, indicating a dose-dependent effect.
- Protein concentration remained higher near the microvessel wall compared to distal regions even after superfusion.
Conclusions:
- Superfusion alters plasma protein distribution in the perimicrovascular interstitial matrix.
- The persistent higher protein concentration near the microvessel suggests increased interstitial gel concentration may impede protein mobility.