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Serum phospholipase A2 in patients after splenectomy
V J Laine1, J M Grönroos, T J Nevalainen
1Department of Pathology, University of Turku, Finland.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|May 1, 1996
Summary
Post-surgery, phospholipase A2 (PLA2) levels rise, but this study found the spleen is not the source of circulating group II PLA2. Elevated group II PLA2 and C-reactive protein indicate inflammation after splenectomy.
Area of Science:
- Biochemistry
- Immunology
- Surgical Research
Background:
- Phospholipase A2 (PLA2) levels elevate in serum during inflammatory conditions, infections, and post-surgery.
- The liver and spleen are suspected sources of circulating PLA2.
Purpose of the Study:
- To investigate the spleen's role as a source of elevated serum PLA2 concentrations after surgical procedures.
- To analyze changes in PLA2 groups I and II, and C-reactive protein (CRP) in patients undergoing splenectomy.
Main Methods:
- Serum samples from patients before and after splenectomy were analyzed.
- Measurements included mass concentrations of group I PLA2, group II PLA2, and CRP.
- Catalytic activity concentration of PLA2 was also determined.
Main Results:
- Postoperative serum showed increased catalytic activity of PLA2 and mass concentrations of group II PLA2 and CRP.
- Group I PLA2 mass concentration remained unchanged.
- Catalytic activity of PLA2 strongly correlated with group II PLA2 mass concentration (r=0.846) and CRP levels (r=0.566).
Conclusions:
- Group II PLA2 is released into circulation following splenectomy.
- The spleen does not appear to be the primary source of circulating group II PLA2 after surgery.