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Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
Published on: December 17, 2011
Lectin binding reveals divergent carbohydrate expression in human and mouse Peyer's patches
R Sharma1, E J van Damme, W J Peumans
1University of Southampton, UK.
Histochemistry and Cell Biology
|June 1, 1996
Summary
Comparing human and mouse gut immune cells reveals significant differences in cell surface carbohydrates. These distinct glycosylation patterns impact how lectins interact with immune cells, highlighting the need for species-specific approaches in gut research.
Area of Science:
- Immunology
- Glycobiology
- Gastroenterology
Background:
- The role of intestinal cell-associated carbohydrates in mediating the transport and processing of lectins by Peyer's patch lymphoid cells is poorly understood.
- Cell surface carbohydrate receptors for lectins can differ significantly across species, necessitating comparative studies.
Purpose of the Study:
- To compare glycoconjugate expression in the follicle-associated epithelium and gut-associated lymphoid tissue of human and mouse intestines.
- To identify species-specific differences in lectin binding patterns on various intestinal immune cells.
Main Methods:
- Utilized a panel of 27 lectins to probe glycoconjugate expression in M-cells, enterocytes, goblet cells, lymphocytes, and macrophages from human and mouse intestines.
- Compared lectin-binding patterns in human and mouse Peyer's patches and associated lymphoid cells.
Main Results:
- Mouse M-cells showed exclusive labeling by fucose-specific lectins, a pattern not observed in human follicle-associated epithelium.
- Distinct lectin binding was observed in human Peyer's patches: Bryonia dioica lectin to T-lymphocytes and Chelidonium majus lectin to B-cells.
- Mannose-specific lectins differentially labeled macrophages in human versus mouse Peyer's patches.
Conclusions:
- Significant differences in glycosylation exist between mouse and human Peyer's patches and their associated lymphoid cells.
- These findings underscore the importance of selecting species-appropriate lectins when studying cell surface glycoconjugates as targets for the gut immune system.
