Related Experiment Videos
Capillary electrophoresis
1Milos V Novotny, Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Current Opinion in Biotechnology
|February 1, 1996
Summary
Capillary electrophoresis is a maturing analytical technique for biomolecules. Advances include laser-induced fluorescence, mass spectrometry, affinity capillary electrophoresis, and microchip integration for sample preparation and separation.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Biophysics
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- CE is increasingly used for analyzing proteins, nucleic acids, and glycoconjugates.
Purpose of the Study:
- To highlight recent advancements in capillary electrophoresis.
- To discuss the integration of ancillary techniques and novel applications.
Main Methods:
- Focus on laser-induced fluorescence detection and mass spectrometry as ancillary techniques.
- Exploration of affinity capillary electrophoresis for binding studies.
- Integration of sample treatment and separation on microchips.
Main Results:
- CE is becoming a major technique for biomolecule analysis.
- Ancillary techniques enhance CE capabilities.
- Affinity CE is valuable for ligand-biomolecule interactions.
- Microchip integration offers novel approaches.
Conclusions:
- Capillary electrophoresis is a rapidly advancing field.
- Integration of advanced detection and sample preparation methods expands CE applications.
- CE is a versatile tool for diverse biomolecular analyses.