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Related Experiment Videos

Capillary electrophoresis

Novotny1

  • 1Milos V Novotny, Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.

Current Opinion in Biotechnology
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

Capillary electrophoresis is a maturing analytical technique for biomolecules. Advances include laser-induced fluorescence, mass spectrometry, affinity capillary electrophoresis, and microchip integration for sample preparation and separation.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biophysics

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • CE is increasingly used for analyzing proteins, nucleic acids, and glycoconjugates.

Purpose of the Study:

  • To highlight recent advancements in capillary electrophoresis.
  • To discuss the integration of ancillary techniques and novel applications.

Main Methods:

  • Focus on laser-induced fluorescence detection and mass spectrometry as ancillary techniques.
  • Exploration of affinity capillary electrophoresis for binding studies.
  • Integration of sample treatment and separation on microchips.

Main Results:

  • CE is becoming a major technique for biomolecule analysis.

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  • Ancillary techniques enhance CE capabilities.
  • Affinity CE is valuable for ligand-biomolecule interactions.
  • Microchip integration offers novel approaches.

    • Conclusions:

    • Capillary electrophoresis is a rapidly advancing field.
    • Integration of advanced detection and sample preparation methods expands CE applications.
    • CE is a versatile tool for diverse biomolecular analyses.