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Simultaneous determination of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acids in human urine by thin
G Alemany1, A Gamundí, C Rosselló
1Dept. de Biologia i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Biomedical Chromatography : BMC
|May 1, 1996
Abstract:
A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.