Engineering new functions and altering existing functions
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Updated: Nov 4, 2026

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay
Published on: October 4, 2024
1Division of Chemistry and Chemical Engineering, 210-41, California Institute of Technology, Pasadena, CA 91125, USA.
Abstract:
Structural and mechanistic information, sequence comparisons, and site-directed mutagenesis data continue to provide a basis for the rational design of new protein functions and the alteration of existing functions. Random mutagenesis and 'directed evolution' approaches, however, are making significant headway in solving protein engineering problems, proving highly practical for tuning properties such as enzyme substrate specificity.
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