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Cellular origin and ultrastructure of membranes induced during poliovirus infection

A Schlegel1, T H Giddings, M S Ladinsky

  • 1Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309, USA.

Journal of Virology
|October 1, 1996
PubMed

Insights

Poliovirus infection induces unique cytoplasmic membranes. These virus-induced structures, formed from host cell organelles, suggest autophagy plays a role in poliovirus replication.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Poliovirus RNA replication occurs on cytoplasmic membranes.
  • Virus-induced membranes are crucial for efficient viral replication.

Purpose of the Study:

  • To investigate the origin and composition of poliovirus-induced membranous structures.
  • To elucidate the role of host cell organelles in viral membrane formation.

Main Methods:

  • Immunoisolation of viral replicative complexes using antibodies against poliovirus protein 2C.
  • Biochemical analysis of isolated membranes.
  • High-pressure freezing electron microscopy.
  • Immunolabeling experiments.

Main Results:

  • Virus-induced membranes are derived from host cell lysosomes, Golgi, and endoplasmic reticulum.
  • Membranes feature double lipid bilayers enclosing cytosolic material.
  • Poliovirus proteins 2C and 3D localize to these membranes alongside cellular markers.

Conclusions:

  • Autophagy or a similar host process is implicated in the formation of poliovirus-induced membranes.
  • These findings provide insights into the mechanisms of viral RNA replication and host-pathogen interactions.

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