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Related Experiment Videos

Experimental melioidosis in hens

A Vesselinova1, H Najdenski, S Nikolova

  • 1Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

Zentralblatt Fur Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B
|August 1, 1996
PubMed
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Hens are susceptible to Pseudomonas pseudomallei, causing generalized melioidosis. This experimental study tracked clinical signs, bacterial spread, and immune responses in infected hens over 30 days.

Area of Science:

  • Veterinary Pathology
  • Infectious Diseases
  • Avian Health

Background:

  • Melioidosis, caused by Pseudomonas pseudomallei, is a significant zoonotic disease.
  • Understanding avian susceptibility is crucial for disease control and public health.
  • Hens represent a potential reservoir for P. pseudomallei transmission.

Purpose of the Study:

  • To investigate the clinical, paraclinical, and pathomorphological outcomes of experimental Pseudomonas pseudomallei infection in hens.
  • To evaluate the immune response, including haemagglutinin titre and macrophage activity, following infection.
  • To determine the course and dissemination of melioidosis in avian hosts.

Main Methods:

  • Experimental intramuscular inoculation of hens with P. pseudomallei (strain 2796).

Related Experiment Videos

  • Monitoring of clinical signs, bacterial load in tissues (spleen, liver), and isolation from egg yolk from 1 to 30 days post-infection.
  • Assessment of haemagglutinin titre, leukocyte counts, and in vitro phagocytic activity of alveolar (aMa) and peritoneal (pMa) macrophages.

    • Main Results:

    • Infection led to leucocytosis and elevated haemagglutinin titres (1:256).
    • Peak bacterial presence in spleen and liver occurred at 1 day post-infection; bacteria were isolated from egg yolk at 15 and 30 days.
    • Maximal leukocyte and pMa phagocytic activity was observed at 3 days post-infection, while aMa activity increased throughout the study. Inflammatory-necrotic changes were noted in viscera and brain.

    Conclusions:

    • Hens are susceptible to P. pseudomallei, exhibiting a generalized subacute course of melioidosis.
    • The study highlights hens as a viable model for experimental melioidosis research.
    • Findings indicate potential for egg contamination and transmission, emphasizing the need for surveillance in poultry.