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Related Experiment Videos

Secretion and processing of apolipoprotein A-I in the avian sciatic nerve during development

M J Lemieux1, C Mezei, W C Breckenridge

  • 1Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.

Journal of Neuroscience Research
|June 15, 1996
PubMed
Summary

Apolipoprotein A-I (apo A-I) synthesis and processing in developing chick sciatic nerves are regulated alongside myelination. Apo A-I secretion increases, with a shift towards acidic isoforms during development.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Developmental Biology

Background:

  • Apolipoprotein A-I (apo A-I) is crucial for lipid transport and has been found in avian sciatic nerves.
  • Apo A-I mRNA and protein levels rise during rapid myelination in avian sciatic nerves.

Purpose of the Study:

  • To investigate the synthesis and processing of apo A-I protein and its isoforms during chick sciatic nerve development.
  • To correlate apo A-I synthesis and processing with myelination stages.

Main Methods:

  • Incubation of endoneurial slices from embryonic and posthatch chick sciatic nerves with [35S]-methionine.
  • Fractionation into secreted and intracellular components to assess labeled apo A-I and Po protein.
  • Analysis of apo A-I isoform processing (acidic vs. basic).

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Main Results:

  • Methionine incorporation into Po protein peaked intracellularly at 15 days embryonic, then decreased with myelin accumulation.
  • Nascent apo A-I levels increased in both secreted and intracellular fractions during development.
  • Apo A-I synthesis increased specifically in the secreted fraction, and secretion shifted towards acidic isoforms with age.

Conclusions:

  • Apo A-I secretion and processing are developmentally regulated in the chick sciatic nerve.
  • These changes occur in parallel with active myelination, suggesting a coordinated biological process.