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Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains
M J Sadowsky1, L L Kinkel, J H Bowers
1Department of Soil, Water, and Climate, University of Minnesota, St. Paul 55108, USA.
Applied and Environmental Microbiology
|September 1, 1996
Abstract:
PCR DNA fingerprinting using repetitive intergenic DNA sequences (rep-PCR) was investigated as a means of differentiating between closely related strains of Streptomyces which were, in some cases, indistinguishable by other classification methods. Our results demonstrated that the majority of strains had unique rep-PCR DNA fingerprints and established that the technique could be a very useful tool in rapidly determining strain identity.