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Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content
N Baskaran1, R P Kandpal, A K Bhargava
1Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
Genome Research
|July 1, 1996
Abstract:
A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.