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Endothelial cell associated anti-elastolytic activity

M E Hanley1, L S Terada, J C Cheronis

  • 1Webb-Waring Institute for Biomedical Research, Department of Medicine at Denver General Hospital, University of Colorado Health Sciences Center, Colorado 80262, USA.

Inflammation
|June 1, 1996
PubMed
Summary
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Bovine pulmonary artery endothelial cells (EC) possess surface anti-elastolytic activity, likely derived from their own protein synthesis. This activity may influence biological processes involving human neutrophil elastase (HNE).

Area of Science:

  • Cell Biology
  • Biochemistry
  • Endothelial Cell Research

Background:

  • Human neutrophil elastase (HNE) plays a role in inflammatory and repair processes.
  • Endothelial cells (EC) are crucial components of blood vessels and interact with circulating factors.

Purpose of the Study:

  • To investigate the presence and nature of anti-elastolytic activity on the surface of bovine pulmonary artery endothelial cells (EC).
  • To determine if EC-associated anti-elastolytic activity is protein-dependent and synthesized by the EC themselves.

Main Methods:

  • Assessing the effect of cultured EC on in vitro human neutrophil elastase (HNE) activity.
  • Treating EC with enzymes (papain, trypsin) or charged molecules (histone, protamine) to probe the nature of the activity.
  • Culturing EC in serum-free media and treating them with cycloheximide to assess the role of protein synthesis.

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Main Results:

  • Addition of EC significantly decreased HNE activity.
  • Enzyme treatment of EC reduced their ability to decrease HNE activity, suggesting a protein basis.
  • EC treated with cycloheximide showed diminished HNE inhibitory activity, indicating EC protein synthesis is involved.
  • EC cultured in serum-free media retained their HNE inhibitory capacity.

Conclusions:

  • Bovine pulmonary artery endothelial cells (EC) possess surface-associated anti-elastolytic activity.
  • This activity is likely due to proteins synthesized by the EC.
  • EC-associated anti-elastolytic activity may modulate biological processes involving HNE, such as inflammation and repair.