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The structural origin of the color differences in the bioluminescence of firefly luciferase
1PRESTO, JRDC, Institute of Physical and Chemical Research (RIKEN), Wako, Japan. yohmiya@postman.riken.go.jp
Abstract:
Six chimeric mutants between Hotaria parvula (lambda max = 568 nm) and Pyrocoelia miyako (lambda max = 550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light-emitting color, five chimeric luciferases could be divided into two groups: the three green-emitting mutants, classified as P. miyako luciferase, and the two yellow-emitting mutants, classified as H. parvula luciferase. Their common fragments between Val-209 and Ala-318 within each group contain the active site for the color differences.
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