Related Experiment Video
Updated: Aug 12, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Electrospray tandem mass spectrometry of alkali metal-containing anionic complexes of tripeptide
1Institute for National Measurements Standards, National Research Council of Canada, Ottawa, Ontario, Canada.
Abstract:
Collision-induced dissociation mass spectra of alkali metal-containing anionic tripeptides are reported. Both N-terminal and C-terminal product ions were observed from the precursor ions [M - 2H + X]- and [M - 3H + 2X]- where X stands for an alkali metal. The results are consistent with precursor ion structures in which an alkali metal ion is centrally located for bonding with the amino terminus, the amide nitrogen atoms, and an oxygen atom of the carboxylate terminus; the second alkali metal ion in [M - 3H + 2X]- is likely attached to the other oxygen atom of the carboxylate terminus.
More Related Videos
12:59A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
Published on: January 8, 2016
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry of Amines
Mass Spectrometry: Amine Fragmentation
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single or...
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example, the fragmentation of...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry