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Partial purification and reconstitution of the tricarboxylate carrier from eel liver mitochondria
V Zara1, G Damiano, M R Franco
1Dept. of Biology, University of Lecce, Italy.
Biochemistry and Molecular Biology International
|May 1, 1996
Abstract:
The tricarboxylate carrier of the inner membrane of eel liver mitochondria has been solubilized with Triton X-100 and partially purified by chromatography of the mitochondrial extract on dry hydroxyapatite. The purified fraction has been reconstituted into liposomes and functionally analyzed. The reconstituted carrier protein catalyzed a 1,2,3-benzenetricarboxylate-sensitive citrate uptake in liposomes. The substrate specificity and the inhibitor sensitivity of the tricarboxylate carrier are similar to those previously determined for the same protein of mammalian mitochondria.