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Antigen-specific IgA-secreting cells induced by peripheral vaccination
J S Rothel1, L A Corner, H F Seow
1University of Melbourne, Molecular Parasitology Laboratory, Werribee, Victoria, Australia.
Immunology and Cell Biology
|June 1, 1996
Summary
Sheep vaccination generates antigen-specific IgA antibody-secreting cells in lymph nodes. This study monitored IgA production following vaccination with a recombinant protein and various adjuvants, revealing significant IgA responses.
Area of Science:
- Immunology
- Vaccinology
Background:
- Humoral immunity is crucial for vaccine efficacy.
- The role of IgA-secreting cells in peripheral lymph nodes post-vaccination requires further elucidation.
Purpose of the Study:
- To investigate the generation and kinetics of antigen-specific IgA-secreting cells in sheep lymph following subcutaneous vaccination.
- To compare the immunogenicity of different adjuvants (incomplete Freund's, Quil A, Al(OH)3) in inducing IgA responses.
Main Methods:
- Sheep prefemoral lymph nodes were cannulated to collect efferent lymph.
- Vaccines containing recombinant Taenia ovis 45W-GST protein with different adjuvants were administered.
- Lymph fluid was analyzed for specific antibodies using ELISA and antibody-secreting cells via ELISPOT assay.
Main Results:
- Significant numbers of antigen-specific IgA-, IgM-, IgG1-, and IgG2-secreting cells were detected in lymph post-vaccination.
- Incomplete Freund's adjuvant elicited a strong IgA response, with IgA-secreting cells constituting up to 11% of specific antibody-secreting cells.
Conclusions:
- Subcutaneous vaccination in sheep induces antigen-specific IgA-secreting cells in peripheral lymph nodes.
- The choice of adjuvant significantly influences the magnitude of the IgA response.