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Ontogenic and glucocorticoid-accelerated expression of rat 14 kDa bile acid-binding protein
Y Z Gong1, T Kato, D A Schwartz
1Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.
Insights
The 14 kDa ileal bile acid-binding protein (I-BABP) and its mRNA are normally expressed in rat ileum around 17 days postnatally. Glucocorticoid treatment accelerates this ileal I-BABP expression.
Area of Science:
- Biochemistry
- Developmental Biology
- Molecular Biology
Background:
- 14 kDa ileal bile acid-binding protein (I-BABP) is a major cytosolic protein in rat ileum.
- This study investigates the normal and glucocorticoid-accelerated postnatal expression of 14 kDa I-BABP.
Purpose of the Study:
- To characterize the developmental expression pattern of 14 kDa I-BABP and its mRNA in rat ileum and ovary.
- To determine the effect of glucocorticoid treatment on the postnatal expression of 14 kDa I-BABP.
Main Methods:
- Detection of 14 kDa I-BABP using antiserum via histochemistry.
- Detection of 14 kDa I-BABP mRNA using antisense cRNA probe via Northern blot analysis.
- Analysis of expression in rats at various postnatal ages (7, 14, 21, 28, 35 days) with and without corticosteroid treatment.
Main Results:
- 14 kDa I-BABP and its mRNA were detected in rat ileum at 21 days and ovary at 35 days of postnatal life.
- Corticosteroid treatment led to earlier detection of 14 kDa I-BABP in the ileum (14 days) compared to controls (21 days).
- Northern blot analysis showed earlier mRNA appearance in corticosteroid-treated rats (11 days) versus controls (17 days).
Conclusions:
- Normal expression of 14 kDa I-BABP and its mRNA occurs in the rat ileum around 17 days postnatally, with later expression in the ovary.
- Glucocorticoid administration results in precocious expression of ileal 14 kDa I-BABP.
- These findings highlight the role of corticosteroids in regulating the developmental expression of I-BABP.
Background:
A 14 kDa protein is a major rat ileal cytosolic bile acid-binding protein (14 kDa I-BABP). This report describes the normal and glucocorticoid-accelerated postnatal expression of 14 kDa I-BABP. METHODS. 14 kDa I-BABP and its mRNA were detected by antiserum and antisense cRNA probe, respectively, in ileum and ovary from 7-, 14-, 21-, 28-, and 35-day-old rats.
Results:
A positive histochemical reaction with 14 kDa I-BABP antiserum was found in cytosol of ileal enterocytes and ovarian luteal cells at 21 and 35 days of age, respectively. Likewise, Northern blot analysis indicated that the cRNA probe hybridized to a single transcript of 500 bp in total cellular RNA prepared from the ileum of 21-day-old and ovary of 35-day-old rats. Corticosteroid treatment resulted in a histochemical reaction in ileum of 14-day-old animals as compared to the appearance of this reaction in 21-day-old control littermates. Similarly, Northern blot analysis detected the earlier appearance of mRNA levels in corticosteroid-treated animals (11 days vs. 17 days in control animals).
Conclusions:
14 kDa I-BABP and its mRNA are normally expressed in rat ileum at 17 days of postnatal life. 14 kDa I-BABP is expressed considerably later in rat ovary. Corticosteroid treatment results in precocious expression of ileal 14 kDa I-BABP.