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Related Experiment Videos

Selective medium for screening for vancomycin-resistant enterococci in faeces

G G Rao1, K Ghanekar, F Ojo

  • 1Lewisham Hospital, NHS Trust, London, UK.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|February 1, 1996
PubMed
Summary

A new selective medium effectively detects vancomycin-resistant enterococci (VRE) in fecal samples. This tool aids in identifying VRE carriers, crucial for controlling the spread of these difficult-to-treat infections.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Vancomycin-resistant enterococci (VRE) infections pose a growing global health threat.
  • Effective detection and isolation of VRE carriers are essential for infection control.

Purpose of the Study:

  • To describe a novel selective medium for detecting fecal carriage of VRE.
  • To evaluate the medium's performance in identifying different VRE resistance phenotypes.

Main Methods:

  • Development and testing of a selective culture medium for VRE.
  • Assessment of medium productivity for VanA, VanB, and VanC VRE phenotypes.
  • Limited clinical trial involving screening of fecal specimens from carriers and contacts.

Main Results:

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  • The medium demonstrated high productivity for VanA (90.4%), moderate for VanB (79.2%), and lower for VanC (65.5%) VRE phenotypes.
  • No vancomycin-susceptible enterococci grew through the medium.
  • In a clinical trial, VRE (Enterococcus faecalis, VanA phenotype) was detected in 4 out of 6 fecal specimens, with nearly pure and easily identifiable growth.

Conclusions:

  • The described selective medium is effective for detecting VRE fecal carriage.
  • The medium shows good specificity, preventing the growth of susceptible strains.
  • This diagnostic tool can aid in identifying VRE carriers for infection control purposes.