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Updated: Aug 19, 2026

In Vitro Transcription Assays and Their Application in Drug Discovery
Published on: September 20, 2016
NusG overexpression inhibits Rho-dependent termination in Escherichia coli
1Institute of Cancer Research, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
Abstract:
The Escherichia coli NusG protein binds Rho in vitro and is required for efficient Rho-dependent transcription termination in vivo. In this work we examine transcription termination in cells that over-express NusG. Termination at the Rho-dependent lambda tL1 and lambda tR1 sites was significantly inhibited by excess NusG, whereas the Rho-independent lambda tl site was fully functional. Although Western analysis showed no reduction in the levels of soluble Rho, termination was restored when Rho was also overexpressed. Our data indicated that the ratio of NusG and Rho proteins affects the efficiency of transcription termination.
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