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Coagulation factor activation in stored platelet concentrates is modulated by the platelets
M I Gyöngyössy-Issa1, T Black, D V Devine
1Canadian Red Cross Society Blood Services, University of British Columbia, Vancouver, Canada.
Vox Sanguinis
|January 1, 1996
Summary
Stored platelet concentrates show activation during storage, impacting platelet viability. Coagulation factors remained stable in platelets but altered in platelet-poor plasma, suggesting platelets may suppress coagulation activation.
Area of Science:
- Transfusion Medicine
- Hematology
- Biochemistry
Background:
- Platelet concentrates (PC) are critical for treating bleeding disorders.
- Understanding coagulation protein behavior during PC storage is essential for optimizing transfusion safety and efficacy.
- Previous studies have not fully elucidated the impact of storage on coagulation within PC.
Purpose of the Study:
- To investigate alterations in coagulation proteins and activation markers within stored platelet concentrates (PC) and platelet-poor plasma (PPP).
- To assess the effect of platelet presence and storage conditions on coagulation dynamics.
- To evaluate the viability and activation state of platelets during storage.
Main Methods:
- Analysis of platelet parameters and coagulation activation markers in PC and PPP at various time points (pre-donation, processing, storage up to 5 days).
- Measurement of activated partial thromboplastin time (aPTT) and prothrombin time (PT).
- Assay of specific coagulation factors (FVII, FXI, FXII), kallikrein activity, and prothrombin F1.2 levels.
- Platelet morphology and surface marker analysis.
Main Results:
- Storage-dependent increases in aPTT and PT were observed in both PC and PPP.
- Coagulation factors FVII, FXI, FXII, kallikrein activity, and prothrombin F1.2 levels remained stable in stored PC.
- Significant alterations in FXII, FVII, kallikrein, and prothrombin F1.2 levels were detected in stored PPP.
- Platelet morphology and surface marker studies indicated platelet activation during storage.
Conclusions:
- Platelet activation occurs during the storage of PC.
- The presence of platelets in storage containers appears to partially suppress coagulation activation.
- This suppression of coagulation comes at a cost to platelet viability.
- Further research is needed to balance coagulation suppression and platelet viability in stored blood products.