Contractile response of the isolated trabecular meshwork and ciliary muscle to cholinergic and adrenergic agents

To characterize the contractile properties of isolated trabecular meshwork strips, we measured the effect of various cholinergic and adrenergic substances on the contractility of trabecular meshwork (TM) strips in comparison with the effects on ciliary muscle (CM) strips. Using an electromagnetic force length transducer we performed measurements of isometric tension on isolated bovine TM and CM strips. Isolated strips were contracted by cholinergic agonists, the relative potency being carbachol > pilocarpine > acetylcholine. The half-maximal effective concentration was 2 x 10(-7) mol l(-1) for carbachol and 2 x 10(-6) mol l(-1) for pilocarpine. To characterize muscarinic receptors, we tested selective antagonists for M1 (pirenzepine) and M3 (4-DAMP). Pharmacologically, the functional muscarinic receptors are of the M3 subtype in TM as well as CM. The M1 subtype seems to be less important. The alpha 1-agonist phenylephrine was more effective in inducing contractions in TM than in CM. The alpha 2-agonist brimonidine induced contractions only in TM. In precontracted tissues the beta-agonist isoproterenol induced a relaxation in both tissues. This relaxation could be inhibited by metipranolol. Epinephrine (or dipivefrin) induced small contractions in TM and CM, which became more prominent, especially in TM, when the beta-adrenoreceptors were inhibited by metipranolol. The data indicate the presence of functional muscarinic, alpha-adrenergic, and beta-adrenergic receptors in bovine TM and CM. The contractile properties of TM and CM are differently modulated by the various drugs. Cholinergic and alpha-adrenergic agonists induced contraction, whereas beta-agonists induced relaxation.