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Applying critical pressure to middle cerebral artery in dogs
1Department of Neurosurgery, Panukkale University School of Medicine, Denizli, Turkey.
Abstract:
The purpose of our model was to provide maximum pressure level of the middle cerebral artery (MCA) without causing any histological variation like focal cerebral ischaemia. In this experimental study 9 dogs were used. A modified Spetzler occluder was applied to the MCA of the dogs to determine regional cerebral blood flow (rCBF) and somatosensory evoked potentials (SSEP). Seven animals underwent the surgical procedure with measurements of rCBF after arterial microdissection and then after applying 80% blood pressure (BP) to the MCA for 15 minutes. In rCBF a 29% average reduction was observed. The average value of rCBF at the end of 15 minutes was 63.5 +/- 6.52 ml/100g min. Simultaneous somatosensory evoked potential recordings were also obtained. When rCBF was reduced 29%, 80% BP applying to MCA did not cause complications.
Insights
This study determined the maximum middle cerebral artery (MCA) pressure without causing cerebral ischemia. Applying 80% blood pressure to the MCA reduced regional cerebral blood flow by 29% but caused no histological complications.
Area of Science:
- Neurology
- Cerebrovascular Research
- Experimental Surgery
Background:
- Focal cerebral ischemia is a critical concern in cerebrovascular research.
- Understanding the limits of middle cerebral artery (MCA) pressure is vital for surgical interventions and treatment strategies.
- Previous research has not precisely defined the threshold of MCA pressure that induces histological changes.
Purpose of the Study:
- To establish the maximum pressure level for the middle cerebral artery (MCA) that does not induce histological variations, specifically focal cerebral ischemia.
- To investigate the impact of reduced regional cerebral blood flow (rCBF) on somatosensory evoked potentials (SSEP).
Main Methods:
- An experimental study involving 9 dogs was conducted.
- A modified Spetzler occluder was used to manipulate MCA pressure.
- Regional cerebral blood flow (rCBF) and somatosensory evoked potentials (SSEP) were measured before and after applying 80% blood pressure to the MCA for 15 minutes.
Main Results:
- A 29% average reduction in regional cerebral blood flow (rCBF) was observed.
- The average rCBF at the end of the 15-minute intervention was 63.5 +/- 6.52 ml/100g/min.
- Applying 80% blood pressure to the MCA, despite a 29% rCBF reduction, did not result in complications or histological changes.
Conclusions:
- The study successfully identified a pressure threshold for the MCA that can be manipulated without causing focal cerebral ischemia.
- Maintaining 80% blood pressure in the MCA for 15 minutes, leading to a 29% rCBF reduction, is safe in the studied model.
- These findings contribute to understanding cerebrovascular autoregulation and may inform clinical practices related to MCA interventions.