Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microglia-derived macrophages in early multiple sclerosis plaques

H Li1, M L Cuzner, J Newcombe

  • 1Multiple Sclerosis Laboratory, Institute of Neurology, London, UK.

Neuropathology and Applied Neurobiology
|June 1, 1996
PubMed
Summary

Microglia are the primary phagocytes in early multiple sclerosis (MS) lesions, clearing myelin debris. This finding is crucial for understanding MS pathogenesis and developing targeted therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenobarbital induction mediated by a distal CYP2B2 sequence in rat liver transiently transfected in situ.

The Journal of biological chemistry·1996
Same author

Induction of phosphoglycerate kinase 1 gene expression by hypoxia. Roles of Arnt and HIF1alpha.

The Journal of biological chemistry·1996
Same author

Identification of novel regions of deletion in familial Wilms' tumor by comparative genomic hybridization.

Cancer research·1996
Same author

Alternatively spliced cyclin C mRNA is widely expressed, cell cycle regulated, and encodes a truncated cyclin box.

Oncogene·1996
Same author

Emergence of preferred structures in a simple model of protein folding.

Science (New York, N.Y.)·1996
Same author

Developmental regulation of collagen differential expression in the rabbit bladder.

The Journal of urology·1996

Area of Science:

  • Neuroimmunology
  • Pathology

Background:

  • Demyelination in multiple sclerosis (MS) involves lipid-laden macrophages in active lesions.
  • The origin of these macrophages (microglial vs. haematogenous) in early MS plaques is unclear.
  • Identifying early phagocyte populations is key to understanding MS pathogenesis.

Purpose of the Study:

  • To determine the origin of phagocytes in early active multiple sclerosis plaques.
  • To differentiate between microglial and haematogenous macrophages in MS lesions.

Main Methods:

  • Utilized histochemistry and immunocytochemistry on active MS plaques.
  • Employed markers such as EBM11 (pan-macrophage), nucleoside diphosphatase (microglial), and non-specific esterase (monocyte/macrophage).
  • Assessed acid phosphatase activity and correlated with oil red O+ foamy macrophages.

Related Experiment Videos

Main Results:

  • In early active MS plaques, 60% of EBM11+ phagocytes showed microglial markers (nucleoside diphosphatase activity).
  • Only 4-15% of EBM11+ phagocytes in early lesions exhibited monocyte/macrophage markers (non-specific esterase).
  • Advanced plaques showed a higher percentage (30-80%) of non-specific esterase+ phagocytes.

Conclusions:

  • Microglia constitute the predominant phagocyte population in the initial stages of demyelination in MS.
  • These findings suggest a significant role for microglia in the early pathogenesis of multiple sclerosis.