Prostanoid modulation of glucose transport in isolated diabetic rat uterus

3-O-14C-methyl-D-Glucose (3-O-MG) transport and 14C-saccharose incorporation were measured in isolated uterine strips from ovariectomized-estrogenized diabetic rats. Glucose transport was decreased in uterine strips from diabetic rats compared with control animals. PGE1 and PGE2 (10(-7) M) stimulated 3-O-MG transport, PGF2 alpha failed to modify this parameter at the same concentration, while insulin (0.5 U/ml) evoked an improvement 30% greater than PGs. In spite of the negative influence exerted by TXA2 over glucose metabolism in the isolated rat uterus, U46619, 10(-5) M (a TXA2 analogue), and OKY064, 10(-7) M (an inhibitor of TXA2 synthesis), failed to modify basal or insulin-treated hexose transport. Neither additive or synergistic interactions between PGE1 or PGE2 (10(-7) M) and insulin at 0.5 U/ml and 0.25 U/ml were detected. We conclude that the stimulatory action of PGE1 and PGE2 on glucose metabolism that has been previously described by us, involves enhancement of glucose transport.