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Growth factor transcripts in rat renal transplants

L C Paul1, K Saito, A Davidoff

  • 1Department of Medicine, University of Toronto at St Michael's Hospital, Ontario, Canada.

American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
|September 1, 1996
PubMed
Summary
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Kidney transplantation and rejection alter growth factor mRNA levels. Chronic rejection shows increased fibroblast growth factor-1 and transforming growth factor-beta 1 transcripts, indicating their role in tissue remodeling.

Area of Science:

  • Nephrology
  • Transplantation Immunology
  • Molecular Biology

Background:

  • Cytokines and growth factors mediate tissue remodeling in renal transplants.
  • Ischemia and rejection episodes impact renal graft integrity.

Purpose of the Study:

  • Investigate mRNA transcript levels of key growth factors in rat kidneys.
  • Analyze changes in platelet-derived growth factor (PDGF)-receptor beta, PDGF-A, PDGF-B, fibroblast growth factor-1, and transforming growth factor beta 1.
  • Correlate growth factor expression with normal kidneys, post-nephrectomy, and rejection states.

Main Methods:

  • Quantitative analysis of mRNA transcript levels.
  • Immunohistochemistry to detect protein expression.
  • Comparison across normal, nephrectomized, and transplanted rat kidneys (syngeneic and allogeneic).

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Main Results:

  • PDGF-receptor beta mRNA increased post-transplantation and in chronic rejection, with protein induction on vascular cells.
  • PDGF-A mRNA increased in early allografts and syngeneic grafts; PDGF-B showed no significant change.
  • Fibroblast growth factor-1 mRNA decreased in acute rejection but increased in chronic rejection.
  • Transforming growth factor-beta 1 transcripts rose in both acute and chronic rejection, with glomerular protein localization.

Conclusions:

  • Renal transplantation and rejection significantly alter intragraft growth factor mRNA levels.
  • Chronic rejection is specifically associated with elevated fibroblast growth factor-1 and transforming growth factor-beta 1 transcripts.
  • These growth factors play a role in the tissue remodeling processes during renal graft rejection.