Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide

O N Jensen1, A Podtelejnikov, M Mann

  • 1Protein & Peptide Group, European Molecular Biology Laboratory, Heidelberg, Germany.

Rapid Communications in Mass Spectrometry : RCM
|January 1, 1996
PubMed
Summary

Delayed ion extraction coupled with MALDI time-of-flight mass spectrometry significantly enhances protein identification. This technique improves database search specificity and accuracy, enabling faster and more comprehensive protein analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perceptions of friendship, peers and influence on adolescent smoking according to tobacco control context: a systematic review and meta-ethnography of qualitative research.

BMC public health·2023
Same author

Sublinear scaling of the cellular proteome with ploidy.

Nature communications·2022
Same author

Site Readiness Framework to Improve Health System Preparedness for a Potential New Alzheimer's Disease Treatment Paradigm.

The journal of prevention of Alzheimer's disease·2022
Same author

Exploring the association between school-based peer networks and smoking according to socioeconomic status and tobacco control context: a systematic review.

BMC public health·2022
Same author

Reconceptualizing ER physician wellness in the midst of the pandemic: survival through the lens of personal agency.

CJEM·2021
Same author

Administration and monitoring of clofazimine for NTM infections in children with and without cystic fibrosis.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2021

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Peptide mass mapping using MALDI is a common method for protein identification via database searches.
  • Existing methods face limitations in specificity and accuracy for complex analyses.

Purpose of the Study:

  • To evaluate the impact of delayed ion-extraction on MALDI time-of-flight mass spectrometry for protein identification.
  • To demonstrate the enhanced specificity and accuracy offered by this improved technique.

Main Methods:

  • Utilized delayed ion-extraction coupled with reflectron MALDI time-of-flight mass spectrometry.
  • Analyzed peptide mass maps generated from protein samples.
  • Performed database searches using the obtained mass accuracy data.

Related Experiment Videos

Main Results:

  • Achieved routine resolution between 6,000 and 12,000, enabling monoisotopic mass assignment.
  • Demonstrated high mass accuracy (<30 ppm over wide range, <5 ppm over narrow range).
  • Observed improved signal-to-noise ratio, allowing assignment of low-intensity peaks and increased sequence coverage.

Conclusions:

  • Delayed ion-extraction significantly enhances the specificity and precision of MALDI-TOF MS for protein identification.
  • This advanced technique facilitates protein identification with fewer peptides and improves the analysis of protein mixtures.
  • Delayed-extraction MALDI-TOF MS represents a powerful tool for robust protein database identification.