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Related Experiment Videos

Fibronectin, mesoderm migration, and gastrulation in Xenopus

R Winklbauer1, R E Keller

  • 1Department of Zoology, University of California, Berkeley 94720, USA. rwinkl@novell.biolan.uni-koeln.de

Developmental Biology
|August 1, 1996
PubMed
Summary

Fibronectin is not essential for mesoderm cell migration or Xenopus gastrulation. It aids cell spreading and protrusion formation, but Xenopus gastrulation proceeds normally even when fibronectin is blocked.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Xenopus laevis Research

Background:

  • Mesoderm cell migration is crucial for gastrulation.
  • A stable interface between migrating cells and the substrate is necessary for migration.
  • Fibronectin's role in this process is not fully understood.

Purpose of the Study:

  • To investigate the role of fibronectin in mesoderm cell migration during Xenopus gastrulation.
  • To determine if fibronectin is essential for maintaining the cell-substrate interface.
  • To elucidate fibronectin's specific contribution to mesoderm cell adhesion and migration.

Main Methods:

  • Examined the role of fibronectin in Xenopus mesoderm cell migration.
  • Assessed the necessity of fibronectin for maintaining the blastocoel roof interface.

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  • Utilized GRGDSP peptide and antibodies to block fibronectin function.

    • Main Results:

    • Fibronectin is not required for maintaining the mesoderm-substrate interface.
    • Fibronectin contributes to, but is not essential for, mesoderm cell adhesion.
    • Fibronectin is necessary for cell spreading and cytoplasmic protrusion formation, controlling protrusive activity.
    • Blocking fibronectin inhibits mesoderm cell migration but does not significantly disrupt gastrulation.

    Conclusions:

    • Fibronectin plays a role in regulating mesoderm cell migration by controlling protrusive activity.
    • Mesoderm cell migration is not essential for normal gastrulation in Xenopus.
    • The stable interface for migration does not depend on fibronectin.