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Novel method for assessing postantibiotic effect by using the Coulter counter

R C Li1, S W Lee, J S Lam

  • 1Department of Pharmacy, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong.

Antimicrobial Agents and Chemotherapy
|July 1, 1996
PubMed
Summary

A new Coulter counter method offers a more efficient way to measure the postantibiotic effect, improving upon the laborious pour plate technique. This advancement reduces errors and material waste in microbiological assays.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Analytical Chemistry

Background:

  • The postantibiotic effect (PAE) is crucial for understanding antibiotic efficacy.
  • Traditional viable cell counting via pour plate technique is standard but inefficient.
  • This method is labor-intensive, time-consuming, and prone to errors from sample dilutions.

Purpose of the Study:

  • To develop and evaluate a more efficient method for measuring the postantibiotic effect.
  • To overcome the limitations of the pour plate technique in PAE studies.

Main Methods:

  • A novel total cell counting technique was developed.
  • A Coulter counter was employed for cell enumeration.
  • Comparison with the conventional pour plate method.

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Main Results:

  • The Coulter counter technique provides a more efficient measurement of PAE.
  • This new method reduces the time and labor required for PAE determination.
  • It minimizes errors associated with sample dilutions and material wastage.

Conclusions:

  • The Coulter counter offers a superior alternative to the pour plate method for PAE studies.
  • This technique enhances efficiency and accuracy in microbiological assays.
  • It represents a significant advancement in the methodology for studying antibiotic effects.