Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombin interaction with platelet membrane glycoprotein Ib

M Jandrot-Perrus1, M C Bouton, F Lanza

  • 1Laboratoire de Recherche sur l'Hémostase et la Thrombose, Faculté de Médecine Xavier Bichat, Paris, France.

Seminars in Thrombosis and Hemostasis
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Description of 18 PNH patients with prevalent GPI-deficient monocytes. An Italian survey.

British journal of haematology·2025
Same author

Overview on novel strategies and current guidelines for hematopoietic stem cell mobilisation and collection.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2023
Same author

[Cultured platelets].

Bulletin de l'Academie nationale de medecine·2020
Same author

Convalescent plasma, an apheresis research project targeting and motivating the fully recovered COVID 19 patients: A rousing message of clinical benefit to both donors and recipients alike.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2020
Same author

WITHDRAWN: Convalescent plasma, an apheresis research project targeting and motivating the fully recovered COVID 19 patients: A rousing message of clinical benefit to both donors and recipients alike.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2020
Same author

NEW HORIZONS ON STEM CELL CRYOPRESERVATION THROUGH THE ARTIFICIAL EYES OF CD 34+, USING MODERN FLOW CYTOMETRY TOOLS.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2020

Platelet glycoprotein Ib (GPIb) is crucial for thrombin receptor activation, enhancing thrombin formation and thrombus development. GPIb binds thrombin via exosite 1, distinct from its von Willebrand factor binding site, promoting platelet responses.

Area of Science:

  • Biochemistry
  • Hematology
  • Molecular Biology

Background:

  • Platelet activation by thrombin is essential for thrombus formation.
  • Glycoprotein Ib (GPIb) is required for low-concentration thrombin responses, as GPIb-deficient platelets show reduced thrombin sensitivity.
  • GPIb acts as the von Willebrand factor (vWF) receptor, mediating platelet adhesion.

Purpose of the Study:

  • To elucidate the mechanism of thrombin binding to platelet glycoprotein Ib (GPIb).
  • To investigate the role of GPIb in thrombin-induced platelet activation.
  • To understand the relationship between GPIb and thrombin receptor (TR) interactions.

Main Methods:

  • Investigated thrombin-GPIb interaction using competitive inhibition assays with hirudin, fibrinogen, and thrombomodulin.

Related Experiment Videos

  • Characterized the GPIb domain involved in thrombin binding.
  • Compared thrombin binding sites on GPIb and TR.

    • Main Results:

    • Thrombin exosite 1, not its catalytic site, is essential for GPIb binding.
    • A hydrophilic domain on GPIb alpha, involving negatively charged residues, mediates ionic interactions with thrombin.
    • The GPIb binding site for thrombin overlaps with the vWF binding site.
    • GPIb and TR bind to distinct subsites within thrombin exosite 1.
    • Soluble GPIb fragments do not inhibit thrombin's hydrolysis of TR, indicating membrane anchorage is key for GPIb's promoting effect.

    Conclusions:

    • Platelet GPIb plays a critical role in amplifying thrombin formation by binding to thrombin's exosite 1.
    • This interaction is distinct from vWF binding and requires GPIb's membrane anchorage to promote thrombin receptor-mediated responses.
    • GPIb-thrombin interaction is vital for efficient platelet activation and thrombus development.