Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human C1q induces eosinophil migration

P Kuna1, M Iyer, E I Peerschke

  • 1Department of Medicine, SUNY-Stony Brook, New York 11794-8161, USA.

Clinical Immunology and Immunopathology
|October 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arerugi = [Allergy]·2023
Same author

Arerugi = [Allergy]·2023
Same author

An Atypical Case of Idiopathic Nonhistaminergic Angioedema With Anti-C1-INH Antibodies.

Journal of investigational allergology & clinical immunology·2022
Same author

Omalizumab substantially improves dermatology-related quality of life in patients with chronic spontaneous urticaria.

Journal of the European Academy of Dermatology and Venereology : JEADV·2017
Same author

Why a registry of Chronic Urticaria (CUR) is needed.

The World Allergy Organization journal·2017
Same author

Mechanisms of action that contribute to efficacy of omalizumab in chronic spontaneous urticaria.

Allergy·2016
Same journal

Mechanisms of virus replication, persistence, and regulation of immune responses.

Clinical immunology and immunopathology·2020
Same journal

Cumulative subject index for volumes 86-89

Clinical immunology and immunopathology·1998
Same journal

D-penicillamine-induced pancreatic islet autoantibody production is independent of the immunogenetic background: a lesson from patients with Wilson's disease.

Clinical immunology and immunopathology·1998
Same journal

Characterization of gastric Na+/I- symporter of the rat.

Clinical immunology and immunopathology·1998
Same journal

CD8(+), radiosensitive T cells of parental origin, oppose cells capable of down-regulating cytotoxicity in murine acute lethal graft-versus-host disease.

Clinical immunology and immunopathology·1998
Same journal

Specific activity of alpha1proteinase inhibitor and alpha2macroglobulin in human serum: application to insulin-dependent diabetes mellitus.

Clinical immunology and immunopathology·1998
See all related articles

Eosinophils express C1q-binding cell surface molecules (C1q-R), including cC1q-R and gC1q-R. These receptors may signal eosinophil locomotion, impacting allergic inflammation and parasitic infection immunity.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Eosinophils (Eo) are crucial in allergic inflammation and parasitic infections.
  • The role of C1q-binding cell surface molecules (C1q-R) on Eo is not well understood.
  • Previous studies suggested C1q-R involvement in IgG-dependent eosinophil-mediated killing.

Purpose of the Study:

  • To immunochemically demonstrate and investigate the biology of C1q-R on eosinophils.
  • To characterize the structure and function of C1q-R on eosinophils.
  • To determine if C1q-R mediates eosinophil migration.

Main Methods:

  • Purification of eosinophils from hypereosinophilic donors.
  • Western blot analysis using antibodies to cC1q-R and gC1q-R.
  • Modified Boyden microchamber assay to assess eosinophil migration induced by C1q, cC1q, and gC1q.

Related Experiment Videos

  • Checkerboard analysis to differentiate chemokinesis and chemotaxis.
  • Assays for eosinophil cationic protein release and CD11b/CD29 expression.

    • Main Results:

    • Western blot confirmed the presence of cC1q-R (60 kDa) and gC1q-R (33 kDa) on eosinophils.
    • cC1q, C1q, and gC1q induced eosinophil migration, primarily through chemokinesis.
    • Eosinophil cationic protein release and CD11b/CD29 expression were not enhanced by C1q or its variants.

    Conclusions:

    • Eosinophils express both cC1q-R and gC1q-R.
    • C1q-R on eosinophils may provide a primary signal for eosinophil locomotion.
    • These findings contribute to understanding eosinophil function in immune responses.