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Analysis of possible WT1 RNA processing in primary Wilms tumors
K B Gunning1, S L Cohn, G E Tomlinson
1Department of Experimental Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, USA.
Oncogene
|September 19, 1996
Summary
Wilms tumor (WT1) RNA processing abnormalities, including editing and splicing, were investigated. Neither WT1 RNA editing at codon 281 nor aberrant exon 2 splicing frequently occurs in primary Wilms tumors.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Wilms tumor (WT1) is a pediatric kidney cancer.
- WT1 RNA processing abnormalities, such as RNA editing and aberrant splicing, are proposed mechanisms for WT1 alteration in tumorigenesis.
- These RNA events can lead to functionally altered WT1 protein without DNA mutations.
Purpose of the Study:
- To investigate the occurrence of WT1 RNA editing at codon 281 and aberrant exon 2 splicing in primary Wilms tumors.
- To determine if these RNA processing events are common mechanisms in Wilms tumor development.
Main Methods:
- Analysis of WT1 mRNA from 15 primary Wilms tumors.
- Sequencing and Southern analysis of DNA from tumors exhibiting aberrant splicing.
Main Results:
- No evidence of WT1 RNA editing at codon 281 was found in the analyzed primary tumors.
- Aberrant splicing of WT1 exon 2 was detected in only one of the 15 primary tumors.
- DNA analysis of the tumor with aberrant splicing showed no alterations in or around exon 2.
Conclusions:
- WT1 RNA editing at codon 281 is not a frequent mechanism in Wilms tumorigenesis.
- Aberrant splicing of WT1 exon 2 is also uncommon in primary Wilms tumors.
- These findings suggest that WT1 RNA processing abnormalities are not major drivers of Wilms tumor development.